Osteocalcin Rabbit Polyclonal Antibody

• Catalog Number: orb10187
• Category: Antibodies
• Description: Osteocalcin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BGP (21-80/100aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 11 kDa
• Target: BGLAP
• UniProt ID: P02818
• RRID: AB_10751546
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Osteocalcin Propeptide; gamma-carboxyglutamic acid
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Chen, Guobao et al. Responses of MSCs to 3D Scaffold Matrix Mechanical Properties under Oscillatory Perfusion Culture ACS Appl Mater Interfaces, 9, 1207-1218 (2017)

PubMed: 28006094

Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Osteocalcin Polyclonal Antibody, Unconjugated (orb10187) at 1:100 followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: bone of mouse embryo, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Osteocalcin Polyclonal Antibody, Unconjugated (orb10187) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.