Osteocalcin Rabbit Polyclonal Antibody

SKU: orb10187

Description

Osteocalcin Rabbit Polyclonal Antibody

Research Area

Osteogenesis

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BGP (21-80/100aa)
• Target: BGLAP
• Molecular Weight: 11 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

OSTCN_HUMAN; BGLAP; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate protein; OC; OCN;

Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Osteocalcin Polyclonal Antibody, Unconjugated (orb10187) at 1:100 followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: bone of mouse embryo, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Osteocalcin Polyclonal Antibody, Unconjugated (orb10187) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

BGLAP

UniProt

P02818

References

Chen, Guobao et al. Responses of MSCs to 3D Scaffold Matrix Mechanical Properties under Oscillatory Perfusion Culture ACS Appl Mater Interfaces, 9, 1207-1218 (2017)

PubMed: 28006094