OspC antibody

• Catalog Number: orb344668
• Category: Antibodies
• Description: OspC antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.
• Concentration: 1.2 mg/mL
• Dilution range: WB: 1:1,000
• Form/Appearance: Lyophilized
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspC protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology. Cross-reactivity with OspC from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q07337
• NCBI: NP_047005.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-OspC Antibody, Borrelia burgdorferi Os
• Note: For research use only
• Application notes: This protein-A purified antibody has been tested for use in Western blotting and in ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

References

Caine, Jennifer A. et al. Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival Cell Microbiol, 19, (2017)

PubMed: 28873507

Western blot analysis of Lane 1: Molecular weight markers. Lane 2: MBP-OspC fusion protein (arrow; expected MW: 63.1 kDa). Lane 3: MBP alone using OspC antibody