OspC Antibody

SKU: orb344668

Description

OspC antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB: 1:1,000
• Reactivity: Bacteria
• Application Notes:
  This protein-A purified antibody has been tested for use in Western blotting and in ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspC protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology. Cross-reactivity with OspC from other sources has not been determined.

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.22 mg/mL
• Disclaimer: For research use only

Alternative Names

rabbit anti-OspC Antibody, Borrelia burgdorferi OspC, PC, Outer Surface Protein C, BB_B19

Western blot showing detection of 0.1 µg of recombinant OspC protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspC fusion protein (arrow; expected MW: 63.1 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n orb348540, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n orb347654) secondary antibody was used at 1:40000 in orb348637 blocking buffer.

Quick Database Links

UniProt

Q07337

RefSeq (Protein):WP_010890595.1

References

Caine, Jennifer A. et al. Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival Cell Microbiol, 19, (2017)

PubMed: 28873507