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ospB Antibody

SKU: orb344676

Description

OspB antibody

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:13,000, WB: 1:1,000
ReactivityBacteria
Application Notes
This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 30.3 kDa in size corresponding to Borrelia burgdorferi OspB protein by Western blotting in the appropriate cell lysate or extract.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenMBP-fusion protein corresponding to Borrelia burgdorferi OspB protein.
TargetospB
PurityThis product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspB protein. A BLAST analysis was used to suggest cross-reactivity with OspB from B. burgdorferi, afzelii, spielmanii, and garinii sources based on 100% homology with the immunizing sequence, and with B. valaisiana based on 99% homology. Cross-reactivity with OspB from other sources has not been determined.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

rabbit anti-OspB Antibody, Outer surface protein B, Borrelia burgdorferi OspB, locus BB_A16

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 890.00
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Free Secondary Antibody (20 ul)0/0

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