OspB antibody

• Catalog Number: orb344676
• Category: Antibodies
• Description: OspB antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspB protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:13,000, WB: 1:1,000
• Form/Appearance: Lyophilized
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspB protein. A BLAST analysis was used to suggest cross-reactivity with OspB from B. burgdorferi, afzelii, spielmanii, and garinii sources based on 100% homology with the immunizing sequence, and with B. valaisiana based on 99% homology. Cross-reactivity with OspB from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P17739
• NCBI: P17739.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-OspB Antibody, Outer surface protein B
• Note: For research use only
• Application notes: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 30.3 kDa in size corresponding to Borrelia burgdorferi OspB protein by Western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Molecular weight markers. Lane 2: MBP-OspB fusion protein (arrow; expected MW = 72.7 kDa). Lane 3: MBP alone using OspB antibody