OspA Antibody

SKU: orb344672

Description

OspA antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: >1:5,000, WB: 1:1,000
• Reactivity: Bacteria
• Application Notes:
  This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 28.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspA protein.
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspA protein. A BLAST analysis was used to suggest cross-reactivity with OspA from B. burgdorferi and sources based on 100% homology with the immunizing sequence. Cross-reactivity with OspA or Osp from other sources has not been determined.

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.12 mg/mL
• Disclaimer: For research use only

Alternative Names

rabbit anti-OspA Antibody, Outer surface protein A, Borrelia burgdorferi OspA, ospA, BB_A15, Plasmid lp54

Western Blot of Anti-OspA Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker. Lane 2: 10 µg HeLa spiked 50 ng OspA. Lane 3: 10 µg HeLa spiked 20 ng OspA. Lane 4: 10 µg HeLa spiked 10 ng OspA. Lane 5: 10 µg HeLa Whole Cell Lysate (p/n orb348668). Lane 6: 10 µg HeLa spiked 50 ng OspB. Lane 7: 10 µg HeLa spiked 50 ng OspC. Primary Antibody: Anti-OspA at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) at 1:70000 for 30 min at RT. Block: BlockOut Buffer (p/n orb348644). Predicted MW: ~28.1kDa OspA protein, 70.5kDa MBP-OspA fusion. Observed MW: ~30kDa, ~75kDa.

Western blot showing detection of 0.1 µg of recombinant OspA protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspA fusion protein (arrow; expected MW: 70.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n orb348540, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n orb347654) secondary antibody was used at 1:40000 in orb348637 blocking buffer.

Quick Database Links

UniProt

P0CL66

RefSeq (Protein):NP_045688.1