You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1248546 |
---|---|
Category | Antibodies |
Description | OSMR Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, WB |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-QIHGEQLDPHVT |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 125kDa band observed in lysates of cell line U937 and approx 70+125kDa in lysates of cell line A431 and HepG2 (calculated MW of 111kDa according to NP_003990.1 (isoform 1) and NP_001310435.1 (isoform 3), and 66.0kDa according to NP_001310436.1 (isoform 4)). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 125kDa |
Target | OSMR |
UniProt ID | Q99650 |
NCBI | NP_003990.1, NP_001161827.1, NP_001310436.1, NP_001310435.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | OSMR, oncostatin M receptor, MGC150626, MGC150627, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 125kDa band observed in lysates of cell line U937 and approx 70+125kDa in lysates of cell line A431 and HepG2 (calculated MW of 111kDa according to NP_003990.1 (isoform 1) and NP_001310435.1 (isoform 3), and 66.0kDa according to NP_001310436.1 (isoform 4)). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248546 (1 ug/ml) staining of U937 (A) and (0.3 ug/ml) A431 (B) and HepG2 (C) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248546 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
Filter by Rating