OSMR Antibody

SKU: orb1248546

Description

OSMR Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 125kDa band observed in lysates of cell line U937 and approx 70+125kDa in lysates of cell line A431 and HepG2 (calculated MW of 111kDa according to NP_003990.1 (isoform 1) and NP_001310435.1 (isoform 3), and 66.0kDa according to NP_001310436.1 (isoform 4)). Recommended concentration: 0.3-1µg/mL. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QIHGEQLDPHVT
• Target: OSMR
• Molecular Weight: Approx 125kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OSMR, oncostatin M receptor, MGC150626, MGC150627, MGC75127, OSMRB

orb1248546 (1 ug/ml) staining of U937 (A) and (0.3 ug/ml) A431 (B) and HepG2 (C) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248546 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

OSMR

UniProt

Q99650

RefSeq (Protein):NP_001310436.1, NP_003990.1, NP_001310435.1, NP_001161827.1