OPA1 Rabbit Polyclonal Antibody

SKU: orb158054

Description

OPA1 Rabbit Polyclonal Antibody

Research Area

CARdiovascular Research, Cell Biology, Mitophagy Fission and Fusion, Neurodegenerative Disease, Neuroscience, Visual System

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human OPA1 (651-750/960aa)
• Target: OPA1
• Molecular Weight: 111 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BERHS; MGM1; MTDPS14; NPG; NTG; largeG; 1200011N24Rik; lilr3; mKIAA0567; OPA1_HUMAN; OPA1; Optic atrophy protein 1; 3.6.5.5; KIAA0567; OPA1_MOUSE; Large GTP-binding protein (LargeG); Optic atrophy protein 1 homolog; OPA1_RAT;

Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-OPA1 (orb158054) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 111 kD, Observed band size: 111 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-OPA1 Polyclonal Antibody, Unconjugated (orb158054) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

OPA1

UniProt

O60313

Kimoto, Saki et al. Nacre extract derived from Pinctada fucata mitigates skeletal muscle aging by suppressing senescence-associated inflammation and preserving contractile features in naturally aged mice and C2C12 myotubes Asian Pacific Journal of Tropical Biomedicine, (2026)

PubMed:

Applications

IF

Reactivity

Mouse