OLIG2 Rabbit Polyclonal Antibody (PE)

• Catalog Number: orb498438
• Category: Antibodies
• Description: OLIG2 Rabbit Polyclonal Antibody (PE)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human OLIG2 (81-180/323aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: ICC/IF=1:100
• Conjugation: PE
• Target: OLIG2
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Basic domain helix loop helix protein class B 1; B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-OLIG2 antibody (orb498438), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.