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OLIG2 Rabbit Polyclonal Antibody (PE)

SKU: orb498438

Description

OLIG2 Rabbit Polyclonal Antibody (PE)

Images & Validation

Tested ApplicationsICC
Dilution rangeICC/IF=1:100-500
ReactivityHuman
Predicted ReactivityBovine, Canine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human OLIG2 (81-180/323aa)
TargetOLIG2
PurificationAffinity purified by Protein A

Storage & Handling

StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

Basic domain helix loop helix protein class B 1; Basic helix loop helix protein class B 1; bHLHB1; bHLHe19; Class B basic helix loop helix protein 1; Class B basic helix-loop-helix protein 1; class E basic helix loop helix protein 19; Class E basic helix-loop-helix protein 19; Human protein kinase C binding protein RACK17; Olig2; OLIG2_HUMAN; Oligo2; Oligodendrocyte lineage transcription factor 2; Oligodendrocyte specific bHLH transcription factor 2; Oligodendrocyte transcription factor 2; PRKCBP2; Protein kinase C binding protein 2; Protein kinase C binding protein RACK17; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; RACK17.

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OLIG2 Rabbit Polyclonal Antibody (PE)

Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-OLIG2 antibody (orb498438), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

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Protocol Information

ICC
Immunocytochemistry
View Protocol

OLIG2 Rabbit Polyclonal Antibody (PE) (orb498438)

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100 μl
$ 540.00
DispatchUsually dispatched within 2-3 weeks
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