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OLIG2 Rabbit Polyclonal Antibody (PE)
Description
Images & Validation
−| Tested Applications | ICC |
|---|---|
| Dilution range | ICC/IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human OLIG2 (81-180/323aa) |
| Target | OLIG2 |
| Purification | Affinity purified by Protein A |
Storage & Handling
−| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-OLIG2 antibody (orb498438), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
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OLIG2 Rabbit Polyclonal Antibody (PE) (orb498438)
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