OLFML1 Rabbit Polyclonal Antibody

• Catalog Number: orb317687
• Category: Antibodies
• Description: OLFML1 Rabbit Polyclonal Antibody
• Target: OLFML1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Canine, Equine, Human, Rabbit, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human OLFML1 (321-402/402aa)
• UniProt ID: Q6UWY5
• MW: 46 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MVAL564; Olfactomedin like 1; Olfactomedin like pr
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (OLFML1) Polyclonal Antibody, Unconjugated (orb317687) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Liver (Mouse) Lysate at 40 ug, Lung (Mouse) Lysate at 40 ug, Eye (Mouse) Lysate at 40 ug, Hippocampus (Mouse) Lysate at 40 ug, Primary: Anti-OLFML1 (orb317687) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kD, Observed band size: 48 kD.