OGT antibody

• Catalog Number: orb340807
• Category: Antibodies
• Description: Rabbit polyclonal antibody to OGT
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human OGT
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:200, IP: 1:20-1:50
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: OGT
• Entrez: 26295, 8473, 108155
• UniProt ID: O15294, P56558, Q8CGY8
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: UDP-N-acetylglucosamine--peptide N-acetylglucosami
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of OGT expression in K562 (A), A549 (B), Hela (C), mouse spleen (D), mouse brain (E) whole cell lysates. (Predicted band size: 74; 103; 115; 116 kD; Observed band size: 117 kD)

Immunohistochemical analysis of OGT staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of OGT staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.