ODC1 Rabbit Polyclonal Antibody

• Catalog Number: orb11186
• Category: Antibodies
• Description: ODC1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine
• Reactivity: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human ODC1 (321-461/461aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 51 kDa
• Target: ODC1
• UniProt ID: P11926
• RRID: AB_10767723
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Ornithine decarboxylase 1; Ornithine decarboxylase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Chen, Shaokui et al. Asparagine improves intestinal integrity, inhibits TLR4 and NOD signaling, and differently regulates p38 and ERK1/2 signaling in weanling piglets after LPS challenge Innate Immun, 22, 577-587 (2016)

PubMed: 27554055

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (orb11186) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (orb11186) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Kidney (Mouse) lysate at 30 ug, Primary: Anti-ODC (orb11186) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

Sample: Kidney (Mouse) lysate at 30 ug, Primary: Anti-ODC (orb11186) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

Sample: Liver (Mouse) lysate at 30 ug, Primary: Anti-ODC (orb11186) at 1:300 dilution, Secondary: AP conjugated Goat Anti-Rabbit IgG at 1:5000 dilution, Predicted band size: 51kD, Observed band size: 51kD.