ODC1 Antibody

• Catalog Number: orb1252723
• Category: Antibodies
• Description: ODC1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ODC1/485
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Rat
• Isotype: IgG1, kappa
• Immunogen: Recombinant human ODC-1 protein was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ODC1
• UniProt ID: P11926
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Ornithine decarboxylase, ODC, ODC1
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin/paraffin human prostate carcinoma stained with ODC-1 antibody.

Western blot testing of human samples using ODC-1 mAb.

Flow analysis of permeablized PC3 cells using ODC-1 antibody.

Immunofluorescence testing of LNCaP cells and Alexa Fluor 488 conjugated ODC-1 antibody.

Formalin/paraffin rat pancreas stained with ODC-1 antibody.

Formalin/paraffin human testicular carcinoma stained with ODC-1 antibody.

SDS-PAGE Analysis of Purified, BSA-Free ODC-1 Antibody (clone ODC1/485). Confirmation of Integrity and Purity of the Antibody.