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Catalog Number | orb1252478 |
---|---|
Category | Antibodies |
Description | ODC1 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | ODC1/487 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human, Rat |
Isotype | IgG2a, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the Ornithine Decarboxylase antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Ornithine Decarboxylase antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | ODC1 |
UniProt ID | P11926 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Ornithine decarboxylase, ODC, ODC1 Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Ornithine Decarboxylase antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IF staining of LNCap cells using AF488 labeled Ornithine Decarboxylase antibody (ODC1/487) (Green). DAPI was used to stain the cell nuclei (blue).
Formalin-fixed, paraffin-embedded rat pancreas stained with Ornithine Decarboxylase antibody (ODC1/487).
SDS-PAGE Analysis of Purified, BSA-Free Ornithine Decarboxylase Antibody (clone ODC1/487). Confirmation of Integrity and Purity of the Antibody.
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Bovine, Canine, Equine, Human, Porcine | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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