ZNF423 Antibody

SKU: orb1246451

Description

ZNF423 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:2000.Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: EPECDQKTSRALEDR
• Target: ZNF423
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ZNF423 , zinc finger protein 423 , Ebfaz, KIAA0760, MGC138520, MGC138522, OAZ, Roaz, ZFP423, Zfp104, OLF-1/EBF associated zinc finger, Smad- and Olf-interacting zinc finger protein, early B-cell factor associated zinc finger protein

orb1246451 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining.

orb1246451 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

ZNF423

UniProt

Q2M1K9

RefSeq (Protein):NP_055884.2