O-GalNAc antibody

• Catalog Number: orb396453
• Category: Antibodies
• Description: Mouse monoclonal antibody against O-GalNAC conjugated to Biotin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9B9
• Tested applications: ELISA, ICC, IHC, WB
• Reactivity: All
• Isotype: IgM
• Immunogen: Synthetic β-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration:

  1 mg/ml

• Dilution range: WB: 1:1000, IF/ICC: 1:50, ELISA: 1:1000, IHC-P: 1:25
• Conjugation: Biotin
• Target: O-GalNAC
• Storage: -20ºC, Conjugated antibodies should be stored at 4ºC
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
• Alternative names: GalNAC antibody, O-GalNAC antibody, β-O-Linked N-A
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-503 was sufficient for detection of O-GlcNAc in 2 µg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Embryonic kidney epithelial cell line (HEK293) using O-GalNAc antibody.

Western Blot analysis of Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA using O-GalNAc antibody.

Immunofluorescence analysis of mouse colon using O-GalNAc antibody.