NUP210 Antibody (N-term)

• Catalog Number: orb1927619
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23675
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IHC-P-Leica: 1:500, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 205111 Da
• Target: This NUP210 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-231 amino acids from the N-terminal region of human NUP210.
• UniProt ID: Q8TEM1
• NCBI: NP_079199.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nuclear pore membrane glycoprotein 210, Nuclear po
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

NUP210 Antibody (N-term) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-NUP210 Antibody (N-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 205 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human lung tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.