NUMB Antibody

• Catalog Number: orb1527924
• Category: Antibodies
• Description: NUMB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 375 and 425
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NUMB
• UniProt ID: P49757
• NCBI: NP_001005743.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: h-Numb; S171; Protein S171; numb homolog; C14orf41
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NUMB by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NUMB antibody orb1527924 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 30 seconds.

Detection of human NUMB by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUMB antibody orb1527924 used for IP at 3 µg/mg lysate.