NUMB Antibody

• Catalog Number: orb234347
• Category: Antibodies
• Description: NUMB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NUMB (194-220aa SFRVTTATEQAEREEIMKQMQDAKKAE), different from the related mouse and rat sequences by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 70804 MW
• UniProt ID: P49757
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein numb homolog;h-Numb;Protein S171;NUMB;
• Note: For research use only
• Application notes: WB: The detection limit for NUMB is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U87 cells using anti-NUMB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of NUMB using anti-NUMB antibody.Lane 1:human HeLa cell;2:monkey COS-7 cell;3:human RT4 cell;4:rat C6 cell;5:mouse NIH/3T3 cell.

IHC analysis of NUMB using anti-NUMB antibody. NUMB was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of NUMB using anti-NUMB antibody. NUMB was detected in a paraffin-embedded section of human lung cancer tissue.