You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb97057 |
---|---|
Category | Antibodies |
Description | Nucleophosmin/NPM1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Nucleophosmin (22-41aa ELKADKDYHFKVDNDENEHQ), identical to the related rat and mouse sequences. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunocytochemistry , 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 32575 MW |
UniProt ID | P06748 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Nucleophosmin;NPM;Nucleolar phosphoprotein B23;Nuc Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Lane 1: Rat Testis Tissue Lysate, Lane 2: Rat Brain Tissue Lysate, Lane 3: HELA Cell Lysate, Lane 4: U87 Cell Lysate, Lane 5: A549 Cell Lysate, Lane 6: SMMC Cell Lysate using NPM1 antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Human Intestinal Cancer Tissue using NPM1 antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Spleen Tissue using NPM1 antibody
Confocal immunofluoroscence analysis of HELA cells using NPM1 antibody
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating