Nucleophosmin/NPM1 Antibody

• Catalog Number: orb251524
• Category: Antibodies
• Description: Nucleophosmin/NPM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Nucleophosmin recombinant protein (Position: M1-L294). Human Nucleophosmin shares 95% amino acid (aa) sequence identity with both mouse and rat Nucleophosmin.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 32575 MW
• UniProt ID: P06748
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Nucleophosmin;NPM;Nucleolar phosphoprotein B23;Nuc
• Note: For research use only
• Application notes: WB: The detection limit for Nucleophosmin is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HL-60 cells using anti-Nucleophosmin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB:Lane 1:human HeLa cell;2:human Jurkat cell;3:human MCF-7 cell;4:rat C6 cell;5:rat NRK cell;6:rat PC-12 cell;7:rat RH35 cell;8:mouse ANA-1 cell;9:mouse RAW264.7 cell;10:mouse Neuro-2a cell;11:mouse HEPA1-6 cell.

IF analysis of Nucleophosmin using anti-Nucleophosmin antibody. Nucleophosmin was detected in an immunocytochemical section of CACO-2 cells.

IF analysis of Nucleophosmin using anti-Nucleophosmin antibody. Nucleophosmin was detected in a paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody. Nucleophosmin was detected in a paraffin-embedded section of Mouse Intestine tissue.

IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody. Nucleophosmin was detected in a paraffin-embedded section of Rat Intestine tissue.

IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody. Nucleophosmin was detected in a paraffin-embedded section of Human Intestinal Cancer tissue.