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Nucleolar protein 3 Rabbit Polyclonal Antibody

SKU: orb100019

Description

Nucleolar protein 3 Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityMouse
Predicted ReactivityBovine, Canine, Human, Rabbit, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from mouse Nucleolar protein 3/Apoptosis repressor with CARD (1-100/220aa)
TargetNOL3
Molecular Weight25 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

Apoptosis repressor with CARD; ARC; Muscle enriched cytoplasmic protein; MYC; Myp; NOP; Nop30; Nucleolar protein 3(apoptosis repressor with CARD domain); Nucleolar protein 3; Nucleolar protein of 30 kDa; NOL3_MOUSE .

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Nucleolar protein 3 Rabbit Polyclonal Antibody

Tissue/cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Nucleolar protein 3 Polyclonal Antibody, Unconjugated (orb100019) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Nucleolar protein 3 Rabbit Polyclonal Antibody (orb100019)

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Available Sizes

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50 μl
$ 240.00
100 μl
$ 390.00
200 μl
$ 510.00
DispatchUsually dispatched within 5-10 working days
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