Nuclear Antigen Antibody

• Catalog Number: orb2642691
• Category: Antibodies
• Description: This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Nuclei of HL60 cells were used as the immunogen for the recombinant Nuclear Marker antibody.
• UniProt ID: Not Known
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Application notes: Optimal dilution of the recombinant Nuclear Marker antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: NM2984R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant Nuclear Marker antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil with Nuclear Marker antibody (clone NM2984R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool prior to testing.

IHC staining of FFPE human tonsil with Nuclear Marker antibody (clone NM2984R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool prior to testing.

SDS-PAGE analysis of purified, BSA-free recombinant Nuclear Marker antibody (clone NM2984R) as confirmation of integrity and purity.