NUB1 Antibody

• Catalog Number: orb1264699
• Category: Antibodies
• Description: NUB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This NYREN18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-117 amino acids from the N-terminal region of human NYREN18.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 71 kDa
• Target: NUB1
• UniProt ID: Q9Y5A7
• NCBI: Q9Y5A7
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: NEDD8 ultimate buster 1, Negative regulator of ubi
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Antibody is used in Western blot to detect NYREN18 in mouse liver lysate.

Western blot analysis of NUB1 using NYREN18 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUB1 gene (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis in SK-BR-3 cell line lysates (35 ug/lane).