NT5DC2 Rabbit Polyclonal Antibody

• Catalog Number: orb312336
• Category: Antibodies
• Description: NT5DC2 Rabbit Polyclonal Antibody
• Target: NT5DC2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Equine, Mouse, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human NT5DC2 (251-350/520aa)
• UniProt ID: Q9H857
• MW: 61 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 5' nucleotidase domain containing 2; 5'-nucleotida
• Note: For research use only

References

Cui, Yuying et al. Decreased RNA?binding protein IGF2BP2 downregulates NT5DC2, which suppresses cell proliferation, and induces cell cycle arrest and apoptosis in diffuse large B?cell lymphoma cells by regulating the p53 signaling pathway Mol Med Rep, 26, 286 (2022)

PubMed: 35894142

Applications

WB

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NT5DC2) Polyclonal Antibody, Unconjugated (orb312336) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HT1080 (Human) Cell Lysate at 30 ug, Primary: Anti-NT5DC2 (orb312336) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kD, Observed band size: 61 kD.