NSE Antibody / Neuron Specific Enolase

• Catalog Number: orb2640228
• Category: Antibodies
• Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.
• UniProt ID: P09104
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.2-0.4ug/ml for 30 min at RT
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: ENO2/1375
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This NSE antibody is available for research use only.
• Note: For research use only
• Entrez: 2026
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human cerebellum with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human pheochromocytoma with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

Western blot testing of human 1) Y79, 2) HeLa and 3) HepG2 cell lysate with NSE antibody (clone ENO2/1375). Predicted molecular weight ~47 kDa.

IHC testing of FFPE mouse pancreas with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1375). Required HIER: boil sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free NSE Antibody (clone ENO2/1375). Confirmation of Integrity and Purity of the Antibody.