NRSF Rabbit Polyclonal Antibody

• Catalog Number: orb13623
• Category: Antibodies
• Description: NRSF Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Porcine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NRSF (401-450/1097aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 121 kDa
• Target: REST
• UniProt ID: Q13127
• RRID: AB_10752534
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: RE1-Silencing Transcription Factor; NRSF; Neural-R
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

NRSF was immunoprecipitated from human hela cells lysate with orb13623 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution. Lane 1: human hela cells lysate 10 µg (Input). Lane 2: orb13623 IP in human hela cells lysate. Lane 3: native rabbit IgG IP in human hela cells lysate (negative control). Secondary, All lanes: Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000.

Sample: Hela (Human) Cell Lysate at 30 ug, Hela KO NRSF (Human) Cell Lysate at 30 ug, Primary: Anti-NRSF (orb13623) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kD, Observed band size: 121 kD.

Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-NRSF (orb13623) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kD, Observed band size: 121 kD.

Tissue/Cell: human gastric tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated (orb13623) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.