NRP1 Antibody (C-term)

• Catalog Number: orb1928128
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22995
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 103134 Da
• Target: This NRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 722-750 amino acids from the C-terminal region of human NRP1.
• UniProt ID: O14786
• NCBI: NP_003864.4, NP_001019799.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Neuropilin-1, Vascular endothelial cell growth fac
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NRP1 Antibody (C-term) in MDA-MB435 cell line lysates (35 ug/lane). NRP1 (arrow) was detected using the purified Pab.

NRP1 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with NRP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.