NRAMP1 Rabbit Polyclonal Antibody

SKU: orb221829

Description

NRAMP1 Rabbit Polyclonal Antibody

Research Area

Inflammation, Macrophage, Vitamins and Minerals

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Human, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NRAMP1 (371-470/550aa)
• Target: SLC11A1
• Molecular Weight: 60 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LSH; NRAMP; NRAMP1; Bcg; Ity; Ity1; Itg; NRAM1_HUMAN; SLC11A1; NRAMP 1; Solute carrier family 11 member 1; NRAM1_MOUSE; NRAM1_RAT;

Tissue/Cell: Rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NRAMP1 Polyclonal Antibody, Unconjugated (orb221829) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SLC11A1

UniProt

P49279