NR1H4 Rabbit Polyclonal Antibody

• Catalog Number: orb156973
• Category: Antibodies
• Description: NR1H4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human FXR/Bile Acid Receptor NR1H4 (175-280/486aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: NR1H4
• UniProt ID: Q96RI1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Bile Acid Receptor NR1H4; BAR; FXR; Farnesoid X ac
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Huang, Fengjie et al. Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism Nat Commun, 10, 4971 (2019)

PubMed: 31672964

Applications

WB

Reactivity

Mouse

Alam, Gulzar et al. Activation of farnesoid X receptor (FXR) induces crystallin zeta expression in mouse medullary collecting duct cells Pflugers Arch, 472, 1631-1641 (2020)

PubMed: 32914211

Applications

WB

Reactivity

Mouse

Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-NR1H4 antibody (orb156973), Dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-NR1H4 antibody (orb156973), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Pancreas tissue lysates, Lane 3: Rat Liver tissue lysates, Lane 4: Human HepG2 cell lysates, Lane 5: Human MCF-7 cell lysates, Primary: Anti-NR1H4 (orb156973) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 50 kDa.