NR1H4 Rabbit Polyclonal Antibody

SKU: orb156973

Description

NR1H4 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/test, ICC/IF=1:50-200
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human FXR/Bile Acid Receptor NR1H4 (175-280/486aa)
• Target: NR1H4
• Molecular Weight: 56 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Bile Acid Receptor NR1H4; BAR; FXR; Farnesoid X activated receptor; Farnesoid X receptor; Farnesoid X-activated receptor; Farnesol receptor HRR 1; Farnesol receptor HRR-1; Farnesol receptor HRR1; FXR; HRR 1; HRR1; NR1H4_HUMAN; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor interacting protein 14; Retinoid X receptor-interacting protein 14; RIP 14; RIP14; RXR interacting protein 14; RXR-interacting protein 14.

Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-NR1H4 antibody (orb156973), Dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-NR1H4 antibody (orb156973), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Pancreas tissue lysates, Lane 3: Rat Liver tissue lysates, Lane 4: Human HepG2 cell lysates, Lane 5: Human MCF-7 cell lysates, Primary: Anti-NR1H4 (orb156973) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

NR1H4

UniProt

Q96RI1

References

Yi-Min Sun 1, Jun-Liang Kuang 1, Hui-Heng Zhang 1, Xi-Xi Xia 1, Jie-Yi Wang 1, Dan Zheng 1, Ke-Jun Zhou 2, Ya-Jun Tang 1, Ai-Hua Zhao 1, Wei Jia 3, Guo-Xiang Xie 2, Xiao-Jiao Zheng Pig bile powder maintains blood glucose homeostasis by promoting glucagon-like peptide-1 secretion via inhibiting farnesoid X receptor World J Diabetes, (2025)

PubMed: 40548276

Applications

IHC

Reactivity

Mouse

Huang, Fengjie et al. Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism Nat Commun, 10, 4971 (2019)

PubMed: 31672964

Applications

WB

Reactivity

Mouse

Alam, Gulzar et al. Activation of farnesoid X receptor (FXR) induces crystallin zeta expression in mouse medullary collecting duct cells Pflugers Arch, 472, 1631-1641 (2020)

PubMed: 32914211

Applications

WB

Reactivity

Mouse