NR1H4 Rabbit Polyclonal Antibody

• Catalog Number: orb574778
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NR1H4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NR1H4
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 54kDa
• Target: NR1H4
• UniProt ID: Q96RI1
• Protein Sequence: Synthetic peptide located within the following region: AECLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCREKT
• NCBI: NP_005114
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: BAR, FXR, HRR1, HRR-1, PFIC5, RIP14
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample Tissue: Human 786-0, Antibody dilution: 1.0 ug/ml.

Sample Type: HepG2 Whole Cell lysates, Antibody dilution: 0.5 ug/ml.

Positive control (+): Human kidney (KI), Negative control (-): Human lung (LU), Antibody concentration: 1 ug/ml.

Human kidney

WB Suggested Anti-NR1H4 Antibody, Positive Control: Lane 1: 50 ug mouse glomerular endothelial lysate, Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti-rabbit-HRP, Secondry Antibody Dilution: 1:5000.

WB Suggested Anti-NR1H4 Antibody Titration: 0.2-1 ug/ml, Positive Control: Human Lung.