NR1D1 Rabbit Polyclonal Antibody

SKU: orb6019

Description

NR1D1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NR1D1 (551-614/614aa)
• Target: NR1D1
• Molecular Weight: 67 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

EAR-1; EAR1; ERBA-RELATED 1; hRev; Nr1d1; NR1D1_HUMAN; Nuclear Receptor Rev-ErbA alpha; Nuclear receptor subfamily 1 group D member 1; Orphan nuclear receptor NR1D1; Rev erbAalpha; Rev erbalpha; Rev-erbA-alpha; Rev-ErbAalpha; REV-ERBalpha; THRA1; THRAL; Thyroid hormone receptor alpha-like; Thyroid hormone receptor, alpha like; V-erbA related protein EAR-1; V-erbA-related protein 1.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-NR1D1 antibody (orb6019), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NR1D1) Polyclonal Antibody, Unconjugated (orb6019) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Sample: BRL-3A (Rat) Cell Lysate at 40 ug, Primary: Anti-NR1D1 (orb6019) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 67 kD, Observed band size: 67 kD.

Tissue/Cell: human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NR1D1 Polyclonal Antibody, Unconjugated (orb6019) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NR1D1/REV-ERB alpha Polyclonal Antibody, Unconjugated (orb6019) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

NR1D1

References

Keng-Liang Kuo 1, Shu-Jyuan Chang 2, Cheng Yu Tsai 3, Yen-Shuo Huang 4, Aij-Lie Kwan 5, Chee-Yin Chai Circadian and autophagy markers correlate with poor prognosis in meningioma patients Adv Med Sci, (2025)

PubMed: 39855471

Applications

IHC

Reactivity

Human

Keng-Liang Kuo 1 2, Shu-Jyuan Chang # 3 4, Aij-Lie Kwan # 1 5, Chee-Yin Chai Correlation between levels of clock protein expression and effects on temozolomide-resistant glioblastoma and tumor progression Hum Cell, (2025)

PubMed: 40123038

Applications

ICC, IHC, WB

Reactivity

Human