NQO1 monoclonal antibody (M01), clone 1E3-A6

• Catalog Number: orb2293630
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant NQO1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1E3-A6
• Tested applications: ELISA, IF, IP, WB
• Reactivity: Human
• Isotype: IgG1 Kappa
• Immunogen: NQO1 (AAH07659, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK
• NCBI: AAH07659
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged NQO1 is approximately 0.3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to NQO1 on HepG2 cell. [antibody concentration 10 ug/ml]

Immunoprecipitation of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NQO1 MaxPab rabbit polyclonal antibody.

NQO1 monoclonal antibody (M01), clone 1E3-A6 Western Blot analysis of NQO1 expression in HepG2.

Western Blot analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody (M01), clone 1E3-A6. Lane 1: NQO1 transfected lysate (30.9 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of NQO1 over-expressed 293 cell line, cotransfected with NQO1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NQO1 monoclonal antibody (M01), clone 1E3-A6 (Cat # orb2293630). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (55.88 KDa).