NPR3 Antibody

• Catalog Number: orb1271012
• Category: Antibodies
• Description: NPR3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This Natriuretic Peptide Receptor C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-97 amino acids from the N-terminal region of human Natriuretic Peptide Receptor C.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:200For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 60 kDa
• Target: NPR3
• UniProt ID: P17342
• NCBI: P17342
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Atrial natriuretic peptide receptor 3, Atrial natr
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:200For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Natriuretic Peptide Receptor C primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Natriuretic Peptide Receptor C mainly to the mainly to the nucleus.

Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody for immunohistochemistry.