NPPA Antibody

• Catalog Number: orb1248813
• Category: Antibodies
• Description: NPPA Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: RIGAQSGLGCNSFR
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 19 kDa
• Target: NPPA
• UniProt ID: P01160
• NCBI: NP_006163.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NPPA, natriuretic peptide A, ANF, ANP, ATFB6, CDD-
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 19kDa band observed in Mouse and Rat Heart lysates (calculated MW of 16.4kDa according to Human precursor NP_006163.1 and 16.6kDa accordiong to Mouse precursor NP_032751.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Heart shows vesicular staining inside the cardiac fibrils, accumulating towards the cell membrane. Recommended concentration, 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248813 (0.01 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248813 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.