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NPPA Antibody

SKU: orb1248813

Description

NPPA Antibody

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Porcine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 19kDa band observed in Mouse and Rat Heart lysates (calculated MW of 16.4kDa according to Human precursor NP_006163.1 and 16.6kDa accordiong to Mouse precursor NP_032751.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Heart shows vesicular staining inside the cardiac fibrils, accumulating towards the cell membrane. Recommended concentration, 5-10ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: RIGAQSGLGCNSFR
TargetNPPA
Molecular WeightApprox 19 kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

NPPA, natriuretic peptide A, ANF, ANP, ATFB6, CDD-ANF, PND, atrial natriuretic factor, atriopeptin, cardionatrin, natriuretic peptide precursor A, prepronatriodilatin

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

NPPA Antibody

orb1248813 (0.01 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NPPA Antibody

orb1248813 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

NPPA Antibody (orb1248813)

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Available Sizes

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0.1 mg
$ 880.00
DispatchUsually dispatched within 5-10 working days
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