NPM1 Antibody

• Catalog Number: orb1526691
• Category: Antibodies
• Description: NPM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Immunogen: between 100 and 150
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-NPM1 antibody A302-403A or A302-404A.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NPM1
• UniProt ID: P06748
• NCBI: NP_002511.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: nucleophosmin (nucleolar phosphoprotein B23, numat
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse NPM1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody orb1526691 used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).