NPHS2 Rabbit Polyclonal Antibody

• Catalog Number: orb1602372
• Category: Antibodies
• Description: NPHS2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human Podocin protein (125-383/383aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: NPHS2
• UniProt ID: Q9NP85
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: nephrosis 2, idiopathic, steroid resistant; NPHS2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Measured by its binding ability in a indirect ELISA. Immobilized Human NPHS2 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human NPHS2 Antibody, the EC50 is 155.5 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human NPHS2 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human NPHS2 Antibody, the EC50 is 374.9 ng/ml.

Sample: Lane 1: Recombinant human Podocin, N-his, Primary: Anti-NPHS2 (orb1602372) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kDa, Observed band size: 30 kDa.