NPHS2 Rabbit Polyclonal Antibody

SKU: orb1602372

Description

NPHS2 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human Podocin protein (125-383/383aa)
• Target: NPHS2
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

nephrosis 2, idiopathic, steroid resistant; NPHS2 gene; PDCN; Podocin; SRN1; PODO_HUMAN.

Measured by its binding ability in a indirect ELISA. Immobilized Human NPHS2 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human NPHS2 Antibody, the EC50 is 155.5 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human NPHS2 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human NPHS2 Antibody, the EC50 is 374.9 ng/ml.

Sample: Lane 1: Recombinant human Podocin, N-his, Primary: Anti-NPHS2 (orb1602372) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kDa, Observed band size: 30 kDa.

Quick Database Links

Gene Symbol

NPHS2

UniProt

Q9NP85