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NPHS2 Rabbit pAb, BF488 conjugated

SKU: orb2399468

Description

NPHS2 Rabbit pAb, BF488 conjugated is a BF488 conjugated antibody targeting NPHS2. This antibody is suitable for IF. It exhibits reactivity with Human, Mouse, Rat samples.

Research Area

Cell Biology

Images & Validation

Tested ApplicationsIF
Dilution RangeIF=1:100-500
ReactivityHuman, Rat
Predicted ReactivityBovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human NPHS2/Podocin (231-330/383aa)
TargetNPHS2
PurificationAffinity purified by Protein A
ConjugationBF488

Storage & Handling

StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

nephrosis 2, idiopathic, steroid resistant; NPHS2 gene; PDCN; Podocin; SRN1; PODO_HUMAN.
Quality Guarantee

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NPHS2 Rabbit pAb, BF488 conjugated

Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-NPHS2 antibody (orb2399468), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

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Protocol Information

IF
Immunofluorescence
View Protocol

NPHS2 Rabbit pAb, BF488 conjugated (orb2399468)

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