NPC2 Antibody

• Catalog Number: orb1257504
• Category: Antibodies
• Description: NPC2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-151 of human NPC2 (NP_006423.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 19kDa
• Target: NPC2
• UniProt ID: P61916
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: NPC2, tissue-specific secretory protein, NP-C2, Ni
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse lung, using NPC2 antibody (orb1257504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human liver damage using NPC2 Antibody (orb1257504) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using NPC2 Antibody (orb1257504) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using NPC2 Antibody (orb1257504) at dilution of 1:100 (40x lens).