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Catalog Number | orb667485 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to NPB |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse |
Immunogen | Recombinant fusion protein of human NPB. The exact sequence is proprietary. |
Dilution range | WB: 1-500-2000 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | NPB |
Entrez | 208990, 256933 |
UniProt ID | Q8K4P1, Q8NG41 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | Anti-PPL7 antibody, anti-PPNPB antibody, anti-Neur Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of NPB expression in SW480 (A), mouse brain (B) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 13 kD)
Immunofluorescent analysis of NPB staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
WB | |
Bovine, Canine, Human, Rat | |
Canine, Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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