NPAS2 Rabbit Polyclonal Antibody

SKU: orb221811

Description

NPAS2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NPAS2 (631-730/824aa)
• Target: NPAS2
• Molecular Weight: 92 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Basic helix loop helix PAS protein MOP4; Basic-helix-loop-helix-PAS protein MOP4; bHLHe9; class E basic helix loop helix protein 9; Class E basic helix-loop-helix protein 9; FLJ23138; Member of PAS protein 4; Member of PAS superfamily 4; MGC71151; MOP4; Neuronal PAS domain containing protein 2; Neuronal PAS domain protein 2; Neuronal PAS domain-containing protein 2; Neuronal PAS2; NPAS2; NPAS2_HUMAN; PAS domain containing protein 4; PAS domain-containing protein 4; PASD4.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Neuronal PAS domain-containing protein 2, NPAS2) Polyclonal Antibody, Unconjugated (orb221811) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Human A549 cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human MCF-7 cell lysates, Primary: Anti-NPAS2 (orb221811) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 92 kDa, Observed band size: 105 kDa.

Quick Database Links

Gene Symbol

NPAS2