NOVA1 antibody

• Catalog Number: orb19796
• Category: Antibodies
• Description: Goat polyclonal antibody to NOVA1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:64000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 51.7, 19.5
• Target: NOVA1
• Entrez: 4857
• Protein Sequence: REMPQNVAKTEPVS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NOVA1 antibody, anti neuro-oncological ventra
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Cerebral Cortex shows staining of selected nuclei. Recommended concentration, 2-4ug/ml.WB: In transfected HEK293 transiently expressing NOVA1 a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19796 (0.01ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing NOVA1 (RC210407) and probed with orb19796 (mock transfection in first lane), tested by Origene.

orb19796 (2.5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.