Notch 1 antibody

• Catalog Number: orb154385
• Category: Antibodies
• Description: Mouse monoclonal antibody to Notch 1 which belongs to the NOTCH family transmembrane receptors that plays an important role in development and the determination of cell fate. It expressed in fetal tissues most abundant in spleen, brain stem and lung. Notch 1 has functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination.
• Clonality: Monoclonal
• Clone Number: mN1A
• Tested applications: FC, ICC, IHC-Fr, IP, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1 kappa
• Immunogen: GST fusion protein containing cdc10-NCR region of mouse Notch1
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Notch1
• Entrez: 4851
• UniProt ID: P46531
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-AOS5 antibody, anti-TAN1 antibody, anti-hN1 a
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of Jurkat cells stained using anti-Notch1 (mN1A) purified antibody (concentration in sample 16 µg/ml, GAM APC, red) from Jurkat cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (intracellular staining).