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Catalog Number | orb1239667 |
---|---|
Category | Antibodies |
Description | NogoA Antibody |
Target | RTN4 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | 1 mg/mL |
Buffer/Preservatives | NogoA Antibody is supplied in PBS containing 0.02% sodium azide. |
Purification | NogoA Antibody is affinity chromatography purified via peptide column. |
Immunogen | NogoA antibody was raised against a 19 amino acid synthetic peptide from near the center of human NogoA.The immunogen is located within amino acids 200 - 250 of NogoA. |
UniProt ID | Q9NQC3 |
MW | Predicted: 131 kDa Observed: ~180 kDa |
Tested applications | ELISA, ICC, IF, IHC-P, WB |
Application notes | NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | NogoA Antibody: ASY, NSP, NOGO, NOGOC, RTN-X, NOGO Read more... |
Note | For research use only |
NCBI | NP_065393 |
Western blot analysis of NogoA in human brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 µg/mL.
Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 2.5 µg/mL.
Immunofluorescence of NogoA in Mouse Brain tissue with NogoA antibody at 20 µg/mL.
Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 5 µg/ml.
Immunohistochemistry of NogoA in mouse brain tissue with NogoA Antibody at 5 µg/mL.
Immunofluorescence of NogoA in mouse brain tissue with NogoA Antibody at 20 µg/mL. Green: NogoA antibody (orb1239667) Red: Phylloidin staining Blue: DAPI staining.
ICC, WB | |
Human, Mouse | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P | |
Mouse | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC | |
Bovine, Canine, Gallus, Mouse, Porcine, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |