Nociceptin receptor Rabbit Polyclonal Antibody

• Catalog Number: orb11143
• Category: Antibodies
• Description: Nociceptin receptor Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Nociceptin receptor (1-47/370aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 40 kDa
• Target: OPRL1
• UniProt ID: P41146
• RRID: AB_10747660
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: H.sapiens mRNA for ORL1 receptor; Kappa type 3 opi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Nociceptin receptor (orb11143) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 52 kD.

Sample: Heart (Rat) lysate at 30 ug, Brain (Rat) lysate at 30 ug, Primary: Anti-Nociceptin receptor (orb11143) at 1:200 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 40kD, Observed band size: 40kD.

Immunofluorescence, Tissue/Cell: human placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated (orb11143) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C.