NMDAR2C Rabbit Polyclonal Antibody

• Catalog Number: orb11162
• Category: Antibodies
• Description: NMDAR2C Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NMDAR2C (601-700/1236aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 132 kDa
• Target: GRIN2C
• UniProt ID: Q14957
• RRID: AB_10762849
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: NR2C; N-Methyl-d-Asprtate receptor 2C; Glutamate R
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-NMDAR2C antibody (orb11162), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells wereincubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-NMDAR2C (orb11162) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 132 kD, Observed band size: 132 kD.