NMDAR2C Rabbit Polyclonal Antibody

SKU: orb11162

Description

NMDAR2C Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NMDAR2C (601-700/1236aa)
• Target: GRIN2C
• Molecular Weight: 132 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GluN2C; NMDAR2C; NR2C; NMDE3_HUMAN; GRIN2C; Glutamate [NMDA] receptor subunit epsilon-3; N-methyl D-aspartate receptor subtype 2C (NMDAR2C | NR2C); NMDE3_MOUSE; NMDE3_RAT;

Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-NMDAR2C antibody (orb11162), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells wereincubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-NMDAR2C (orb11162) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 132 kD, Observed band size: 132 kD.

Quick Database Links

Gene Symbol

GRIN2C

UniProt

Q14957