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Catalog Number | orb1273487 |
---|---|
Category | Antibodies |
Description | NMDA NR2A Antibody |
Target | Grin2a |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Reactivity | Human, Rat |
Form/Appearance | Lyophilized |
Concentration | batch dependent |
Purification | Affinity Purified |
Immunogen | NMDA NR2A polyclonal antibody was raised against a C-terminal fusion protein of the NR2A subunit of the NMDAR. |
UniProt ID | Q00959 |
MW | 180 |
Tested applications | IHC, IP, WB |
Application notes | NMDA NR2A Subunit antibody is specific for the 180k NR2A subunit in Western blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the C-terminal fusion protein of the NR2A subunit that was used to generate the antibody. No reactivity towards the NR2B subunit of the NMDAR. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB, and 3 μg/200 μg lysate will quantitatively precipitate all NMDAR 2A for IP. |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
NCBI | Q00959 |
NMDA NR2A Subunit Antibody
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |