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Catalog Number | orb1294254 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to NLRP3 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the N-terminus region. |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200–1:300 Immunohistochemistry (Paraffin) 1:100 – 1:200 |
Conjugation | Unconjugated |
Target | NLRP3 |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-NLRP3 antibody - at 1/700 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294254 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NLRP3 at 1/100.
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294254 anti-NLRP3 antibody (red) at 1:200 and 4°C.
IHC-P | |
Canine, Equine, Porcine, Sheep | |
Human, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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