NLRP3 Antibody

• Catalog Number: orb1238795
• Category: Antibodies
• Description: NLRP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-NALP3 antibody (orb1238795) was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3. The immunogen is located within amino acids 120 - 170 of NALP3.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 118 kDa Observed: 118 kDa
• Target: NLRP3
• UniProt ID: Q96P20
• NCBI: NP_899632
• Storage: NALP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: NALP3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: NALP3 Antibody: AII, AVP, FCU, MWS, FCAS, CIAS1, N
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Validation of NALP3 in Human K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 labeling NALP3 with orb1238795 at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).