NLGN1 Antibody

• Catalog Number: orb1247209
• Category: Antibodies
• Description: NLGN1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-TAKQDDPKQQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 150kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa according to NP_055747.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the membranes/ cytoplasm of U251 and U2OS cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NLGN1
• UniProt ID: Q8N2Q7
• NCBI: NP_055747.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NLGN1, KIAA1070, MGC45115, neuroligin 1, OTTHUMP00
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 150kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa according to NP_055747.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the membranes/ cytoplasm of U251 and U2OS cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247209 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane/cytoplasmic staining.

orb1247209 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane/cytoplasmic staining.