NLE1 Rabbit Polyclonal Antibody

• Catalog Number: orb157995
• Category: Antibodies
• Description: NLE1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Equine, Human, Porcine, Rabbit, Rat, Sheep
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NLE1 (351-450/485aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 53 kDa
• Target: NLE1
• UniProt ID: Q9NVX2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: FLJ10458; NLE 1; Nle; Notchless gene homolog; Notc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ren, Zhaozhou et al. Knockdown of NLE1 inhibits development of malignant melanoma in vitro and in vivo NLE1 promotes development of malignant melanoma Exp Cell Res, 404, 112636 (2021)

PubMed: 34019907

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-NLE1 (orb157995) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD.

Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-NLE1 (orb157995) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD.