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Nkx2.6 Rabbit Polyclonal Antibody
Catalog Number: orb221765
| Catalog Number | orb221765 |
|---|---|
| Category | Antibodies |
| Description | Nkx2.6 Rabbit Polyclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC-Fr, IHC-P |
| Predicted Reactivity | Human |
| Reactivity | Mouse |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Nkx2.6 (1-100/301aa) |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 32 kDa |
| Target | NKX2-6 |
| UniProt ID | A6NCS4 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | CSX2; Homeobox protein NK-2 homolog F; homeobox pr Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Nkx2.6) Polyclonal Antibody, Unconjugated (orb221765) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
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